FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45329505
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG488910.10785690247444794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA9400.014.12397737
ACCCGGC66450.011.6795125
CGTAACG18250.010.35699942
ATAGCGT13500.010.00117136
AGTAACG57800.09.88719525
ATAGGGG103800.09.64692238
TACGGTT29200.09.55490135
TCACCCG87000.09.3602523
CCGGACA87200.09.33883512
CACCCGG89850.09.2636444
ACGGCGA62300.09.20971529
ACGGACA83350.08.90767818
CGGACAC89550.08.86775213
TTACGGT32850.08.69879334
ATAGCTC82750.08.67436739
GGCATAG116400.08.6405635
GGTCACT160900.08.6121467
AAGCCGG282500.08.55393645
GTAACGG65550.08.5466126
AACGGCG67100.08.517328
Produced by FastQC (version 0.11.5)