FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51610692
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG661020.12807811218652135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG39750.014.48673626
GGGAGGT347400.014.2144373
GGAGGTC356850.013.6867094
AGCTTAG252500.013.22160545
ATAGGGG264650.012.92338238
GGCATAG262900.012.87241635
GTCACTC374500.012.4023048
GGTCACT377350.012.3779397
GAGGTCA395650.012.3729325
ACTCTCC390750.012.00735411
TGGCATA288150.011.76004834
AGGTCAC418950.011.7062896
GAGCTTA286300.011.60798644
TGTCGTT51250.011.32589828
ATATAGC59450.011.2768896
CACTCTC410700.011.27615210
TCACTCT421550.011.2261289
TGGGAGG332450.010.8271972
GTCGTTC55400.010.7617529
GCTAAGT58600.010.36758914
Produced by FastQC (version 0.11.5)