FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52604262
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA772300.1468131992803169No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG740500.14076806172093054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28950.013.36648126
GGGAGGT219500.012.8427463
ATAGGGG172800.012.8271738
AGGGATC210100.012.2497632
GGCATAG175150.012.14036135
GGAGGTC233650.011.6990834
AGCTTAG184050.011.64981645
GAGCTTA197550.010.80909144
CAGGGAT265600.010.6467021
TGGCATA203100.010.61361734
GTCACTC248450.010.5774238
CATAGGG208350.010.32510737
ACTCTCC255950.010.32110411
GCATAGG212450.010.05123336
GAGGTCA276150.09.9966975
GGGATCA266400.09.7456973
CACTCTC272750.09.52862910
GACGCAA52150.09.49223244
TCACTCT287100.09.4050539
TAATCGG48650.09.3413544
Produced by FastQC (version 0.11.5)