FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45547923
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1001470.21987171621415097No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG519860.11413473233455673No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT478300.10501027675839357No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT472010.10362931367913308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29300.023.8749146
CGGTATA43050.020.63793820
GTTCGAC34500.020.4079155
GCCGGCT264600.019.907841
CCGGCTT311800.019.3710652
TTCACGC329950.017.9423037
TCACGCT334100.017.645388
AGACCGC126050.017.5065084
TCGACGA41850.016.9299457
CGGCTTC375250.016.7666053
CGACGAG40850.016.738718
CAGACCG132900.016.7226583
GTCCGTT39400.015.3627391
TTCGGCG99250.015.33218844
GTTCCTA71700.015.27744615
ACGCTCA394100.015.10163210
CTACACG168600.015.02957645
CCGTACG103250.014.9453954
AACGCTA386450.014.86520623
ACGCTAC388300.014.80604924
Produced by FastQC (version 0.11.5)