FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45547923
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG654550.14370578434498538No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG601730.13210920726286465No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA547020.12009768261002811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT280400.015.10570053
GGAGGTC315100.013.449224
GGTCACT313450.013.2846337
GTTGTCG27150.013.10167426
GTCACTC323700.012.8577128
GAGGTCA339300.012.725745
ACTCTCC332200.012.57825111
AGGTCAC346100.012.4904066
TCAGACG108500.012.1631572
TCACTCT351950.011.9666969
TGGGAGG281500.011.9319152
AGCTTAG269000.011.90240745
CACTCTC355200.011.70025110
GGCATAG276000.011.479938535
ATAGGGG283700.011.41934938
TGGCATA298800.010.71972234
CTGGGAG353400.010.6712511
GAGCTTA307400.010.31923844
TAGCGTA6450.010.14433337
TATCCTA96950.010.1314855
Produced by FastQC (version 0.11.5)