FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51268833
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG183450.011.8920545
GGGAGGT289900.011.7384043
ATAGGGG200850.010.86484238
GGAGGTC312000.010.827484
GGCATAG204250.010.74961935
GTTGTCG22450.010.72314926
GAGCTTA218050.010.13021744
AGGGATC185900.09.8915492
GGTCACT334300.09.66021257
ACTCTCC343100.09.59601611
GTCACTC339550.09.5903838
TGGCATA230550.09.55275934
ATCCTAG99000.09.2062896
TCAGACG83450.09.1701042
TAGGCTA74400.09.0740610
TATAGCG67500.09.0018377
TATCCTA84100.08.9646725
CAGGGAT226900.08.9183251
CGTGGCG115500.08.8672211
ATATAGC40600.08.7578166
Produced by FastQC (version 0.11.5)