FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47280165
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1135750.24021701277903745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC225150.024.1569794
GACCGCG226400.023.8245685
ACCGCGT227600.023.759196
CAGACCG237100.023.043983
CGCGTTC237850.022.6691028
CCGCGTT238500.022.607327
GAACGAT243000.022.45126245
TCAGACC274050.020.3316022
AGAACGA280350.019.50535244
GCGTTCT281100.019.3012719
CTCAGAC324900.018.5776791
AATACGG297000.018.28354532
TACGGAG301200.017.99100534
ATACGGA302100.017.9823333
AAGAACG318550.017.19454843
ACGGAGA322150.016.84885835
CAATACG324900.016.8246231
CGTTCTC336650.016.5173110
CGGAGAG327700.016.4811336
GCACTAC90400.016.37452525
Produced by FastQC (version 0.11.5)