FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47280165
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT269800.013.8338613
AGCTTAG177050.013.66314445
ATAGGGG181350.013.39869538
GGCATAG183350.013.14257535
GGAGGTC277850.013.1224384
GTTGTCG21200.012.411880526
TGGCATA199700.012.19042434
GAGCTTA200250.011.98942344
ACTCTCC318000.011.33696911
GAGGTCA348650.010.7520515
GTCACTC338950.010.6697388
CATAGGG229000.010.61064537
AGGTCAC358450.010.2904866
GGTCACT358800.09.9669447
TGGGAGG257400.09.9499782
GCATAGG245250.09.90775336
CACTCTC367800.09.81436210
TAGGGGA362800.09.71794739
GCTCTGT380050.09.40923922
TCTCCCC390650.09.30925913
Produced by FastQC (version 0.11.5)