FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43854919
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT542480.12369878051764271No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG489540.11162715863185153No Hit
GTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC469240.106998259419884No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG461880.10531999842480612No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA441870.10075722634443814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC134650.019.5645474
CAGACCG142900.018.6396943
ACCGCGT147050.017.8229736
GTCCGTT34600.017.6918011
GACCGCG153250.017.0431965
CCGCGTT158150.016.5578167
CGCGTTC160700.016.2810788
GAACGAT171500.015.61002645
CCCCCCT36500.015.4143921
TCAGACC181650.015.3693562
CTCCGAT37100.015.3470841
CCCGTAT26900.014.8917931
CCGGCTT228450.014.4562012
TTCACGC185300.014.3988757
GCGTTCT183100.014.3384379
TCACGCT187250.014.1528138
TGTCGCT161850.014.0399536
ATGTCGC161150.013.93427635
CCGTACG48900.013.89368254
ACGCTCA194650.013.81124310
Produced by FastQC (version 0.11.5)