FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50868229
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG777400.15282623658865732No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT634360.12470652359452106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG15600.019.4671926
GCCGGCT257500.016.8751721
ACGTCCG24900.016.53481332
TCACGCT274900.016.480718
CCGGCTT305000.016.2638192
GTCCGTT39300.015.8609931
CGTTACG18900.015.7160821
TTCACGC302600.015.2248217
AGACCGC210350.015.2185334
GAACGAT210250.014.99583545
CAGACCG218750.014.7885183
TACGGTA99600.014.63554844
CGGCTTC344650.014.5885813
ACGCTCA318450.014.51649410
CCCGTAT44200.014.1535611
CCCCCCT44000.014.0644641
ACGGTAC103150.014.01484545
CGAACGA30750.013.901319532
AACGCTA320350.013.75043723
TTACGGT105750.013.74185743
Produced by FastQC (version 0.11.5)