FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50868229
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519650.10215610219101592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT29650.013.198194511
GTTGTCG31850.013.06782926
ACCCGGC93750.013.0577455
TCACCCG98250.012.7363983
AGCTTAG181500.011.8656345
TCGTGTA34950.011.38993314
GGGAGGT318650.011.2513923
GGCATAG194850.011.08711735
ACACGGA111650.010.67560616
CGGACAC125350.010.6220713
GGTCACT283550.010.5242537
GTCACTC289100.010.4310228
GGAGGTC303250.010.4188284
GTCGTGT39750.010.41100113
CACCCGG123900.010.3527584
ATAGGGG216350.010.31821938
CCGGACA133150.010.26962412
ATAGCTC110450.010.22801739
GACACGG116700.010.2140315
GAGCTTA211550.010.17962344
Produced by FastQC (version 0.11.5)