FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54806436
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT151150.023.78756745
AGACCGC167900.021.4956474
GACCGCG168500.021.3922025
ACCGCGT173100.020.8366646
CAGACCG175350.020.6465343
CCGCGTT182600.019.7529147
AGAACGA186700.019.3424844
CGCGTTC187050.019.3191078
AATACGG188700.019.1249932
ATACGGG102350.018.64132333
TACGGGG111300.017.56687534
AAGAACG208650.017.40470143
CAATACG220300.016.57571831
TTCGGCG62500.016.09195944
TCAGACC231200.015.9705112
GTCCGTT42950.015.928361
CGTACGC64500.015.7329345
AACGCTA112700.015.69191923
CCGTACG63700.015.6834064
GCGTTCT233700.015.6072199
Produced by FastQC (version 0.11.5)