FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54806436
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27300.015.08100626
AGCTTAG176000.015.0590645
ATAGGGG179150.014.92232938
GGCATAG183800.014.44593835
GGGAGGT281400.013.223893
GAGCTTA205850.013.02962344
CATAGGG208800.012.77071937
TGGCATA212500.012.61133134
GGAGGTC296400.012.4256264
GCATAGG216650.012.25554436
GTCACTC298250.012.1908868
ACTCTCC314350.011.6174711
GGTCACT311600.011.43022257
TAGGGGA330000.010.69228139
AGGTCAC354900.010.5554676
GAGGTCA367450.010.2987165
CACTCTC367850.010.11745410
TGTCGTT42800.09.88263228
TCACTCT397100.09.6215339
TGGGAGG307650.09.5869422
Produced by FastQC (version 0.11.5)