FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52825943
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC98400.018.7473264
CAGACCG104900.017.950253
GACCGCG104050.017.729335
GTCCGTT41150.016.808321
ACCCGTC36150.016.62180132
ACCGCGT111500.016.5850776
ACGTTCG21750.016.4459296
TCGTATG39650.015.7742145
CCGCGTT123350.015.2281857
GAACGAT123100.015.00483245
CGTTACG18500.014.957121
CCGTACG95100.014.87958054
CGCGTTC127450.014.7026128
TCGGCGC96350.014.64070145
TTCGACG15400.014.4622236
TTCGGCG99050.014.28764744
CGTACGC101500.014.0521835
CGATTAT64100.014.0385593
CTCCGAT51400.013.8947871
ACCGCGA21450.013.74010545
Produced by FastQC (version 0.11.5)