FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51459660
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1239080.24078666668221282No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT721760.14025743660179643No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC645080.12535644425167208No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT594800.11558568400957177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21950.022.1389716
GAACGAT310000.021.75933845
AGACCGC298500.020.9300234
TCACGCT338600.020.9215958
GCCGGCT301700.020.8616681
CAGACCG303850.020.6132723
AACGCTA337750.020.3760223
ACGCTAC341100.020.18361724
CGCTACC345750.019.63363525
GAAAACG345800.019.60818920
AAAACGC353450.019.52749621
GATCGCG25750.019.4834485
CCGGCTT383450.018.9744992
AAACGCT365850.018.87195222
CGGTATA29800.018.72210320
ATACGGG149700.018.65687433
TTCACGC387000.018.4736217
AATACGG365350.018.44749332
AGAACGA375700.018.01447744
TACGGGG155150.017.9441134
Produced by FastQC (version 0.11.5)