FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50508675
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA527240.10438602873664772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT275950.012.1587283
AGCTTAG190100.011.8968545
GGCATAG192200.011.68546135
ATAGGGG194150.011.62728538
GCGTCGT26700.011.37565111
GTCACTC272250.011.0968878
GGAGGTC289450.010.9250724
GAGCTTA211900.010.72633644
GAGGTCA303100.010.6782135
GTTGTCG23250.010.54927826
ACTCTCC289200.010.51019711
TGGCATA222450.010.27835434
AGGTCAC311250.010.1675786
GGTCACT300200.010.1453097
TCACTCT311050.09.9593069
CACTCTC305700.09.83963510
CATAGGG236600.09.5887137
CACCGAA350400.09.51128145
TCGTGTA33150.09.36639714
GCATAGG242900.09.32975636
Produced by FastQC (version 0.11.5)