FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42795196
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18800.018.7880656
AGACCGC107650.018.2866544
CGTTACG15850.017.606221
CAGACCG117350.016.8900533
GACCGCG123350.015.8314545
ACCGCGT129050.015.1844956
CCGTACG33150.015.1342734
GTCCGTT30600.014.4951281
CCGCGTT136650.014.3399867
CCCGGAT111850.014.2116991
CGTACGC35650.014.1360715
CGCGTTC139200.014.1096188
GCCGGCT157150.014.0980161
CGGTATA32400.013.61350720
CCGGCTT182400.013.5553612
TCGACGA27800.013.5148717
ACGTTCG9250.013.3770756
CCCGTAT24150.013.33202651
CGGATAC53450.013.2166123
TTCACGC170150.013.1694487
Produced by FastQC (version 0.11.5)