FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56646015
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT667500.11783706232468427No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC644360.1137520441640952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC163750.023.6056654
CAGACCG176900.022.1180153
GACCGCG180050.021.1439365
GAACGAT189650.020.47731845
ACCGCGT192150.019.9530546
CCGCGTT200000.019.1923967
CGCGTTC202850.018.9782078
AGAACGA222800.017.62241444
GCGTTCT222000.017.4526089
ATACGGA228150.017.21867433
TACGGAG226750.017.20592934
AATACGG226850.017.1983332
AGACGCT299000.016.83368541
CCCCGCA286600.016.78453635
CTCAGAC282300.016.498241
CCGCAGA317300.016.33771937
TCAGACC254500.016.2139852
CCGTACG53200.016.0288354
CCCCCGC315100.015.80904334
CGTACGC54500.015.7292185
Produced by FastQC (version 0.11.5)