FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49995169
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT1160850.2321924344330149No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT998550.19972929784475776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT354700.029.02429445
AGAACGA435950.023.63095744
AGACCGC441800.023.4345054
CAGACCG453300.022.9299833
GACCGCG461850.022.4220075
ACCGCGT469350.022.1546766
CGCGTTC478250.021.7278638
CCGCGTT479150.021.7062447
AATACGG479650.021.52965432
ATACGGA226200.021.14895633
CAATACG491950.021.08695231
AAGAACG488700.021.03419743
TACGGAG227350.021.0024134
TCAGACC508300.020.6790332
GCGTTCT503150.020.6347269
TTCGGCG109100.020.41985544
ATACGGG273200.020.34391633
TACGGGG276600.020.0531934
CTCAGAC553900.019.942151
CCCAATA535250.019.3434729
Produced by FastQC (version 0.11.5)