FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49995169
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG590550.11812141289091352No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA510670.10214386914063638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG182900.015.76917545
GGCATAG201350.014.43615635
ATAGGGG202750.014.35859638
GGGAGGT324300.013.8352683
GAGCTTA222450.013.07681744
TGGCATA225650.012.911734
GGAGGTC355850.012.53331854
CATAGGG246050.011.78572737
ACTCTCC372050.011.74395311
GTCACTC381400.011.5157328
GCATAGG259250.011.15964336
CACCCGG116250.010.9882911
AGCATAG126700.010.84923845
GGTCACT407400.010.7039247
CACTCTC411850.010.69682110
TGGGAGG300350.010.5243952
TCTCCCC418200.010.35614913
TGTCCAA430750.010.25319526
GCTCTGT428750.010.10652822
TAGGGGA427600.09.9597139
Produced by FastQC (version 0.11.5)