FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49203947
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33900.020.1664876
AACGCTA147600.019.64326723
ACGCTAC151450.019.15899524
CGCTACC158000.018.33638225
CGTTACG29400.017.45626821
AAAACGC171600.017.10504321
TCACGCT178750.017.0219048
CGGTATA48900.017.02119420
GAAAACG175000.016.80098320
GAACGAT129900.016.7806945
TTCACGC190300.016.2251287
AGACCGC135350.016.0423074
TCGACGA42900.015.7260737
AAACGCT188250.015.68827222
CAGACCG141100.015.5003673
GACCGCG140750.015.3948235
ACGCTCA198400.015.29067410
ACCGCGT143150.015.2383086
CCGCGTT146100.014.8998377
GCCGGCT154200.014.8356021
Produced by FastQC (version 0.11.5)