FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46027494
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG658510.14306883620472582No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA473450.10286243261473241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29050.017.04981626
GGGAGGT306350.015.1473253
GGAGGTC328500.014.1258974
ATAGGGG249000.013.22716938
AGCTTAG251350.013.18438345
GGTCACT356900.012.9893537
GGCATAG256000.012.95660435
GAGGTCA371400.012.9337775
GTCACTC365150.012.6659378
TGTCGTT38600.012.6567528
ACTCTCC368250.012.47005511
TGGGAGG283200.012.21772
TCACTCT383500.012.1952659
CACTCTC383450.012.00477410
GAGCTTA280800.011.88049744
ATATAGC50250.011.7092656
TGGCATA287600.011.69285634
AGGTCAC487400.010.9938386
GCTAAGT47050.010.71306614
CTGGGAG367600.010.4306461
Produced by FastQC (version 0.11.5)