FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49658964
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG108500.012.36312345
ATAGGGG117500.011.64435138
GGCATAG118400.011.251327535
CATAGGG133400.010.20574537
GGGAGGT211050.09.7746653
TCAGACG87100.09.76318552
GAGCTTA138700.09.7036144
TGGCATA141700.09.6394134
GTCACTC209550.09.4900698
GCATAGG142400.09.48177136
GGTCACT216250.09.0399377
GTTGTCG17050.08.84116426
ACTCTCC226100.08.7762611
GGAGGTC237000.08.5809324
CGTGGCG121100.08.4711191
CGTAGGG65150.08.18613837
GCGTAGG65850.08.16752236
GGCGTAG66650.08.136744535
CAGACGT98150.08.1139223
TAGGGGA236450.08.0230539
Produced by FastQC (version 0.11.5)