FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50488794
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG143750.014.17935345
GGCATAG152300.013.30972735
ACCCGGC87500.013.0384635
ATAGCTC84200.012.56050539
ATAGGGG165400.012.47534538
GAGCTTA167500.012.25154944
GGTCACT252250.011.8462157
TGGCATA173400.011.78097934
GGGAGGT269250.011.7769093
GTCACTC258400.011.5997258
CATAGGG177300.011.57376537
TCACCCG104250.011.4413133
TAGCTCT95250.011.0797340
TCAGACG87400.011.072422
CGGACAG106500.010.64767619
GCATAGG195800.010.53693336
GGAGGTC296800.010.3117334
ACTCTCC292200.010.18860411
CCGGACA117550.010.18412412
ACGGACA122900.09.83121318
Produced by FastQC (version 0.11.5)