FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47188002
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG573940.12162837494157942No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA533710.1131029027251461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG128000.013.48710845
GGCATAG135350.012.99532835
ATAGGGG140750.012.62420538
AGGGATC153050.011.726152
GAGCTTA152900.011.56859644
TGGCATA154450.011.44628734
ATAGCTC72350.011.410459539
ACCCGGC78550.011.3758355
GGGAGGT241300.011.0823713
CATAGGG160550.011.01136837
TATCCTA92500.010.8282255
GGAGGTC240000.010.7574224
ATCCTAG107700.010.6095496
TAGCTCT80600.010.21460740
CAGGGAT202850.010.1872341
CGGGACG57450.010.17676217
CCGGACA93400.010.13627812
TCACCCG93200.010.0956423
GCATAGG176950.010.05459836
AGCGCTC72850.010.0344059
Produced by FastQC (version 0.11.5)