FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56983039
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1156280.202916520475505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG49500.027.455026
TCGACGA57350.023.7727437
CGGTATA83400.022.12014220
CGACGAG62300.021.5227788
GTTCGAC64500.021.209675
GACCGCG311250.020.6678265
AGACCGC313250.020.586154
ACCGCGT314800.020.4990846
CAGACCG320450.020.242983
CCGCGTT330000.019.5458167
GAACGAT333150.019.41013745
CGCGTTC337350.019.1199658
CGTTACG26450.018.37249821
TCAGACC369250.017.963752
GCGTTCT381550.016.9581139
CTCAGAC436800.016.5736371
GTTCCTA100200.016.57023815
GTCCGTT42050.016.3579651
AGTTCGA85900.016.3186764
ATAGAGT125600.015.99565124
Produced by FastQC (version 0.11.5)