FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56983039
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG604900.10615439446815043No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG575260.10095284668829264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG42700.015.33371226
GGGAGGT323500.013.4416223
AGCTTAG248250.012.50788445
GGAGGTC337650.012.4985674
GGCATAG256250.012.03831435
ATAGGGG266450.011.85609238
AACTCTA138150.011.46610145
TGTCGTT58050.011.395370528
GAGCTTA277650.011.167232544
GCTAAGT59350.011.14426914
TGGCATA281050.011.1124334
GTCGTTC61250.010.68982529
ATATAGC73400.010.6347666
GCGTCGT35350.010.56435311
ACTCTCC394000.010.54617211
TATAGCT74350.010.4080867
GGTCACT396000.010.281987
TCGTGTA38750.010.10188714
TCGTTCC65750.010.02665130
CATAGGG318400.09.87925737
Produced by FastQC (version 0.11.5)