FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48108864
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32300.011.97993926
AGCTTAG134400.010.59495845
ACCCGGC79650.010.479335
GGCATAG136300.010.46358635
ATAGGGG139350.010.2835438
TGTCGTT40200.09.84950528
ATATAGC55450.09.4123486
GGGAGGT236550.09.3116253
GGTCACT214150.09.1492597
GAGCTTA154150.09.13527444
GTCACTC220500.09.1305448
CATAGGG157000.08.98418137
ATAGCTC86150.08.79994239
GGAGGTC230750.08.7461224
TGGCATA167450.08.664844534
ACTCTCC233200.08.66222711
GCATAGG165150.08.6089336
GTCGTTC48100.08.51244129
CCGGACA96700.08.49081712
GAGGTCA248900.08.433395
Produced by FastQC (version 0.11.5)