FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51526899
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT696220.1351177760571231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT160450.022.06776845
AGACCGC163900.021.6247634
CAGACCG176500.020.195693
GACCGCG175500.020.1569925
ACCGCGT183150.019.376456
AGAACGA191900.018.52147344
CCGCGTT191700.018.477017
CGCGTTC196850.017.9593418
TACGGAG201000.017.58862334
AATACGG205350.017.33657532
ATACGGA208750.017.05420533
TCAGACC228600.015.967012
CCGTACG75700.015.8345384
CAATACG229550.015.67544131
CGTACGC78750.015.4782855
ACGGAGA230700.015.39264735
CTCAGAC273850.015.3404041
TTCGGCG78600.015.28312344
CCCGTAT35300.015.2007571
AAGAACG235950.015.054111543
Produced by FastQC (version 0.11.5)