FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51526899
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC81950.011.752345
GGCATAG107400.011.37477535
AGCTTAG109600.011.24859445
ATAGGGG113650.010.94898538
TCACCCG92400.010.8142583
CATAGGG118300.010.327661537
TAGCGTA6900.010.10879837
GTTGTCG17550.09.99792526
GAGCTTA128450.09.73977944
GGGAGGT221650.09.7372563
ATAGCTC90300.09.693542539
GCATAGG126900.09.66263636
CACCCGG106000.09.341374
TGGCATA133050.09.24953134
CGGACAG102800.09.03922519
GTCACTC198850.09.0306348
TCAGACG85500.08.9232852
TAGCTCT99500.08.91033140
GACACGG108650.08.88457915
ACGGACA109700.08.86051518
Produced by FastQC (version 0.11.5)