FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54174641
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2020900.37303431323153574No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG610490.11268925621491428No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA556520.1027270305307607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT325100.029.72460245
AGAACGA405350.023.9230744
AGACCGC434050.022.4785294
CAGACCG442750.022.1079793
GACCGCG450900.021.618555
ACCGCGT457600.021.3266076
CGCGTTC459950.021.220548
CCGCGTT460950.021.1745017
AAGAACG472450.020.49677343
AATACGG476800.020.2579432
ATACGGA478650.020.14703633
TACGGAG479700.020.05603234
TCAGACC494100.020.0109232
TTCGACG29050.019.4422236
CAATACG500750.019.38785231
GCGTTCT508950.019.2304749
CTCAGAC544900.019.0763821
ACGGAGA511800.018.80691535
CCCAATA526600.018.5686529
CGGAGAG522800.018.31225636
Produced by FastQC (version 0.11.5)