FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54174641
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG186500.014.11669545
ATAGGGG194550.013.51083538
GGCATAG195050.013.2109435
GTTGTCG29300.012.747375526
GGTCACT270550.012.37247857
CATAGGG217200.012.05029637
GAGCTTA218650.012.010344544
GGGAGGT303800.011.8833613
TGGCATA220450.011.84201734
GTCACTC294200.011.7682558
GCATAGG227100.011.55461936
ACTCTCC296000.011.4387511
GAGGTCA320050.011.006615
GGAGGTC320650.010.9508974
AGGTCAC330050.010.775466
TCACTCT336750.010.26133259
CACTCTC333150.010.19003310
TGTCGTT42850.09.3992828
TGGGAGG309400.09.3123132
TAGGGGA362450.09.30736139
Produced by FastQC (version 0.11.5)