FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54710783
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG868170.15868352679214992No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG717620.13116609937752124No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC698610.12769146440474083No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC640250.11702446298383265No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG632670.11563899569852619No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG621960.11368142912522382No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC614660.1123471400509841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT226600.020.6730291
AACGCTA237650.019.89067323
CCGGCTT255150.019.7969682
ACGCTAC240300.019.69987724
TTCACGC251300.019.5248417
CGCTACC245950.019.20271325
TCACGCT255250.019.1257448
AAAACGC256900.018.48689521
CGTTACG20400.018.41730721
GAAAACG258550.018.28136420
AAACGCT268250.017.69649722
CGGCTTC303250.017.5989953
TTCGACG17650.017.0800066
ACGCTCA290600.017.04711210
CGAACGA25300.016.3644232
ATGTCGC252600.016.30097435
CACGCTC306650.015.9494079
AGACCGC87100.015.6282814
TGTCGCT268400.015.41734936
GTCCGTT35450.015.303571
Produced by FastQC (version 0.11.5)