FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49033819
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1773220.3616320401231648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT299550.028.37605945
AGAACGA365800.023.32300444
AGACCGC371650.023.2774644
CAGACCG380550.022.7922613
GACCGCG386900.022.359965
ACCGCGT393450.021.8962256
CCGCGTT399700.021.5412437
CGCGTTC404250.021.3210478
AAGAACG399850.021.2973643
TCAGACC435900.020.2192042
AATACGG425100.020.0583832
GCGTTCT431500.019.97989
ATACGGA426100.019.97962633
TACGGAG428400.019.88823534
TTCGACG20450.019.8039866
CAATACG441050.019.34319731
CTCAGAC481750.019.1951481
ACGGAGA457950.018.64952735
CGGAGAG464550.018.28379436
CCCAATA472050.018.1683629
Produced by FastQC (version 0.11.5)