FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49033819
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26150.014.19453126
ATAGGGG149350.013.43865638
AGCTTAG150300.013.08022245
GGCATAG153300.012.85627335
GGGAGGT235700.012.4331733
GGTCACT242250.011.3508047
CATAGGG178250.011.28461737
GAGCTTA179200.011.0861544
TGGCATA181750.011.01712534
GTCACTC254800.010.9860038
GGAGGTC264650.010.9537664
GCATAGG188850.010.63899136
ACTCTCC267700.010.57410211
TGTCGTT37150.010.5368628
CACTCTC293750.09.66708110
GAGGTCA309900.09.462225
AGGTCAC310600.09.4182646
TCACTCT317050.09.3114989
AGGGATC150100.09.1780142
TAGGGGA302550.09.11036939
Produced by FastQC (version 0.11.5)