FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42902069
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG5587681.302426696484032No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3664610.854180249442049No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT2937450.6846872583231358No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC1935230.45108080917962257No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG1523340.35507378443682985No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT1485780.3463189619130024No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG1409840.32861818389225006No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1277030.29766163492021797No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1236870.2883007810182768No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG1147390.26744397805150144No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT963920.22467914076591505No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC759310.17698680219828092No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC759260.17697514774870182No Hit
GCCGGCTTCACGCTCAGGAGAAAACACTACCTCTCTTCCTCGTGGTTTTCG752270.1753458556975422No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC721220.1681084425089149No Hit
CTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCTA567010.1321637891170237No Hit
GGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCT550230.12825255583827438No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT549090.12798683438787067No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGT529290.12337167235454309No Hit
CACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTA521330.12151628398154876No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG499480.11642328951547767No Hit
GCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTT493370.11499911577691044No Hit
CCCTCGTCCAACCAGCTGCCACGTCCTGTAACCAAAAGTGACCGGGATGAA453410.1056848796732857No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA451220.10517441478172067No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCG442440.10312789343562895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT1098750.028.4407371
CCGGCTT1361150.025.4139652
CGGTATA46950.025.12562620
TTCGACG39350.025.0433436
CGGCTTC1471900.023.8685843
GTTCGAC41350.023.342365
GGCTTCA1540150.022.9321234
TTCACGC1538650.022.780477
TCACGCT1538750.022.7658318
TCGACGA43800.022.3448837
GAACGAT799600.021.93682145
GCTTCAC1650350.021.6094445
CGACGAG44450.021.3095028
CACGCTC1667850.021.0238769
TCGGCGC458350.020.786245
AGACCGC856600.020.7785994
ACGCTCA1701200.020.77832410
CAGACCG859250.020.7145183
AGTTCGA48750.020.3990824
ACCGCGT874550.020.3418316
Produced by FastQC (version 0.11.5)