FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43143909
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT494230.11455382960315441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC105600.022.6896154
ACCGCGT111100.021.6061926
CAGACCG114150.021.226643
GACCGCG114000.020.9778185
CCGCGTT119050.020.2010387
CGCGTTC118850.020.1593158
GAACGAT140400.017.1451845
TCAGACC148750.016.6986662
GCGTTCT145750.016.6238849
CCCGTAT27300.016.4489611
TTCGACG13700.015.9286536
CTCAGAC194600.015.3066141
CCCCGCA127350.015.15705135
CGAACGA46100.014.98196832
AGAACGA160050.014.94183544
ATACGGA161050.014.82123933
AATACGG160200.014.81567232
CCGTACG39150.014.5963214
AAGCGGC133100.014.53914528
CTCCGTT57500.014.3635341
Produced by FastQC (version 0.11.5)