FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43143909
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG147150.014.8312938
GTTGTCG17950.014.66694526
GGCATAG143500.014.58149335
AGCTTAG151250.014.04245
GGGAGGT203850.014.0291543
GGAGGTC206550.013.4535814
GAGCTTA162800.013.05952144
TGGCATA166450.012.74679234
CATAGGG174400.012.1398337
GCATAGG183300.011.55038936
ACCCGGC43950.010.9553155
GGTCACT267300.010.2343637
GTCACTC269600.010.1472288
TAGGGGA269400.09.90471539
TGGGAGG226450.09.9007822
GTAACGA43900.09.63545818
CTGGGAG274000.09.58785251
ACTCTCC292200.09.53199311
ATCGGTT50250.09.2674236
CGTAACG12650.09.2483842
Produced by FastQC (version 0.11.5)