FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45053093
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG789170.1751644443146223No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG571030.1267460149739331No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA516220.11458036854428619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG34200.018.02624526
AGCTTAG236450.016.0533745
GGGAGGT356250.015.1833853
ATAGGGG255650.015.05853238
GGCATAG262300.014.45379735
GGAGGTC372450.014.3900664
GGTCACT370250.014.1410667
GTCACTC375300.013.986718
GAGCTTA274450.013.89616544
GAGGTCA394450.013.6502245
TGGCATA282950.013.48641434
ACTCTCC395300.013.38729411
TGTCGTT47450.013.22966328
ATATAGC56300.013.1882266
GCTAAGT48300.012.95030214
CACTCTC411600.012.67674210
TGGGAGG318450.012.6756492
GTCGTTC50100.012.66462129
TCACTCT419600.012.6548749
AGGTCAC431300.012.41068656
Produced by FastQC (version 0.11.5)