FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50538636
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2645200.5234015417432318No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA575810.1139346143018185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT384450.032.81638345
AGAACGA459400.027.47226344
ACCGCGT501350.026.9735016
CCGCGTT506500.026.7081227
AGACCGC507750.026.6605954
GACCGCG507450.026.6538755
CGCGTTC507800.026.6441788
CAGACCG520100.026.0654893
TCAGACC551850.024.7324242
AAGAACG516150.024.45204243
AATACGG549450.024.38259132
GCGTTCT562100.024.1593069
TACGGAG557300.024.02987934
ATACGGA558600.023.99421533
CAATACG570600.023.53849231
CTCAGAC610850.023.1121031
ACGGAGA578700.023.08268435
CGGAGAG585850.022.73935736
CCCAATA597000.022.6047929
CGTTCTC628750.021.75880610
Produced by FastQC (version 0.11.5)