FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50538636
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG23750.013.0735726
AGCTTAG149450.012.5708145
ATAGGGG153750.012.18943838
ACCCGGC69950.012.1255735
AGGGATC141900.012.0433292
GGCATAG157700.011.86956135
GGGAGGT249200.011.36230853
ATAGCTC72850.011.14885139
GGAGGTC257950.010.8505454
TGGCATA175000.010.82469834
GAGCTTA174800.010.76054144
CAGGGAT190100.010.4267851
CATAGGG183050.010.36126837
ACGGACA84400.010.3438318
TAGCTCT80550.09.97160440
TAGGCTA72050.09.8972510
TCGCGCT34250.09.85379145
TCACCCG98900.09.7405053
TCAGACG75800.09.7132762
GCATAGG198100.09.57368836
Produced by FastQC (version 0.11.5)