FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45712304
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG562290.12300626982179677No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA471180.10307509330529478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT221650.011.6738853
GTTGTCG29550.011.49744826
GGCATAG179650.011.49735435
ATAGGGG185650.011.319674538
AGCTTAG187050.011.17487945
GGAGGTC231100.011.0602194
TCAGACG84000.010.9018562
AGGGATC189250.010.6764232
CGTGGCG144400.010.377491
GAGCTTA201200.010.35540144
TGGCATA204550.010.08677434
ACTAACC180200.09.95143138
AACTAAC183900.09.812386537
CAGACGT109750.09.7791061
GGGATCA200850.09.4996883
ATATAGC45550.09.4840726
CAGGGAT233350.09.3529631
ATAATCG79750.09.2539783
GTAACGA71700.09.16316918
AATCGGT82050.09.1316175
Produced by FastQC (version 0.11.5)