FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57352050
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1376930.24008383309750916No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT762330.1329211423131344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT319050.023.0158331
TCACGCT400550.020.1259048
TTCGACG28550.019.859116
CCGGCTT425000.019.4771292
CGTTACG34650.019.28473321
AACGCTA408450.018.76694923
ACGCTAC410800.018.67054624
ACGCTCA443950.018.33066710
AGACCGC139350.018.3130554
TTCACGC444050.018.2354017
CGCTACC425550.017.97053325
CGGCTTC473900.017.9497473
AAAACGC430600.017.94265421
CAGACCG144700.017.7758713
GAAAACG435750.017.65314720
AAACGCT446000.017.28779622
CACGCTC469750.017.1898489
CCGTACG165500.017.0239774
TTCGGCG161550.016.85205344
TCGGCGC162750.016.76959645
Produced by FastQC (version 0.11.5)