FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57352050
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG612560.10680699294968532No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA595660.10386028049564051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG233050.013.29265145
GGCATAG235700.013.1779735
GGGAGGT355200.012.696253
TCAGACG174250.012.6948952
GGTCACT347500.012.2162677
GTCACTC348650.012.1565298
ATAGGGG260950.012.06818938
TGGCATA261000.012.03844334
ACTCTCC359600.012.01432611
GAGCTTA261500.011.92331544
TAGCGTA9550.011.77619537
GGAGGTC377100.011.6481734
ACCCGGC85500.011.0372095
CACTCTC384500.011.03680710
TCACTCT394450.010.8942239
GAGGTCA417150.010.646975
CATAGGG298000.010.53676937
CAGACGT211500.010.352383
CACCGAA371700.010.31344545
GCATAGG308550.010.21237236
Produced by FastQC (version 0.11.5)