FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46587758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT855250.1835782696389897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC198950.021.3842744
CAGACCG215150.019.9100573
ACCGCGT221600.019.2894576
GAACGAT220350.019.26631745
GACCGCG220950.019.2346125
CCGCGTT232550.018.3715087
CGCGTTC235600.018.1337138
TTCGACG14100.017.2322046
GTCCGTT32000.017.0223941
TCAGACC257000.016.9480272
GCGTTCT256400.016.706529
AGAACGA260350.016.35796444
CTCAGAC315850.015.3219081
TACGGAG279300.015.18584734
ATACGGA283050.015.1356833
CCGGCTT229800.015.03793052
TTCACGC204850.014.8483067
AATACGG288600.014.80619832
TCACGCT204700.014.727338
AGCGCTA26900.014.63868723
Produced by FastQC (version 0.11.5)