FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46587758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG602790.1293880679984643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG188200.015.47021145
GGGAGGT292150.014.3017293
ATAGGGG211800.013.94828638
GGCATAG210350.013.87330635
GGAGGTC306800.013.3034544
GAGCTTA223650.013.02814144
TGGCATA232100.012.65079734
GTTGTCG22500.012.29996126
CATAGGG258450.011.53511337
TGGGAGG275600.011.4215912
GCATAGG266800.011.14880236
ACTCTCC385450.010.57722611
TCAGACG73700.010.4716422
TCTCCCC387200.010.41324613
CTGGGAG343250.010.3045761
GCTCTGT395250.010.2239222
GTCACTC408100.010.0232478
TGTCCAA408550.09.96264226
TCTGTCC411700.09.93017824
GTCAGGA156450.09.9090451
Produced by FastQC (version 0.11.5)