FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46004681
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1583030.34410194040906406No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT917630.19946448492926186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC193100.022.8843354
CAGACCG201350.022.1031283
GACCGCG219050.020.2554915
GACGGCA374300.019.83764838
TGACGGC384950.019.27713437
AGTTGCA390800.019.05643744
ACGGCAG392200.018.97813639
CGGCAGT402350.018.62238940
ACCGCGT241650.018.4816026
TTCGGCG73350.018.18958744
CTGACGG420800.017.67759736
TCAGACC262350.017.358412
CAGTTGC433400.017.26644943
CCGTACG79100.017.0384254
GTCCGTT39550.016.9670121
CGTACGC79100.016.8962025
AACGATC446550.016.6973821
CTCAGAC295400.016.5037651
CCGCGTT275550.016.2078727
TCGGCGC84250.015.86289545
Produced by FastQC (version 0.11.5)