FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51189043
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1436310.28058934409068753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT240500.028.98301545
AGAACGA295200.023.70453344
AGACCGC307000.022.6443794
ACCGCGT311000.022.3603676
GACCGCG313100.022.2103925
CCGCGTT317650.021.926237
CGCGTTC322050.021.6126968
CAGACCG324200.021.5054663
AATACGG335500.020.7795332
TACGGAG341350.020.3734834
ATACGGA343950.020.29067833
AAGAACG346850.020.17721743
CAATACG355750.019.67193231
TCAGACC363300.019.4263272
ACGGAGA365550.019.09843835
GCGTTCT369200.018.9683599
TTCGGCG89400.018.7753844
TTCGACG19250.018.4657386
CTCAGAC417000.018.1859681
CGGAGAG383500.018.15143836
Produced by FastQC (version 0.11.5)