FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51189043
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG164200.014.91075745
ATAGGGG171750.014.23152438
GGCATAG171050.014.18258335
GAGCTTA191050.012.95686144
GGGAGGT311350.012.8423953
TGGCATA196600.012.55677534
GTTGTCG21850.012.25495826
GGAGGTC313650.012.1834144
CATAGGG203750.012.09586537
GTCACTC318300.011.7250528
ACTCTCC323250.011.65136311
GGTCACT317800.011.6433737
GCATAGG218900.011.24694636
ACCCGGC75800.010.8306765
TAGGGGA350900.010.36516839
AGCATAG121050.010.13154545
AGGTCAC373350.010.1032126
CACTCTC380400.09.91828710
ATAGCTC75500.09.86732339
TCTCCCC390650.09.75085113
Produced by FastQC (version 0.11.5)