FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52778284
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1399840.26523029812791943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT285750.027.39085245
AGACCGC335400.024.021824
CAGACCG343800.023.5592863
AGAACGA343650.022.8348344
GACCGCG351900.022.8059445
ACCGCGT367400.021.9050396
CCGCGTT375150.021.452137
CGCGTTC377200.021.347478
AAGAACG376050.020.87325543
GCGTTCT401100.020.1091149
TCAGACC410550.020.0531792
TACGGAG397000.019.97111334
ATACGGA401300.019.92066833
AATACGG403750.019.80535932
CTCAGAC445800.019.5602531
CAATACG419800.019.10174831
ACGGAGA427500.018.65161735
CGGAGAG434350.018.27483236
CCCAATA457950.017.54488229
ACTCCCC463650.017.33356725
Produced by FastQC (version 0.11.5)